After repeated offences, the province announced they will take Church of God (Restoration) to court, which will select the fine amount.
The information was included in the government issued COVID-19 Enforcement update released Tuesday, where they described the action as "sworn an information under the legislation to a business for repeated offences".
The church, located about 15 kilometres south of Steinbach held numerous services, violating public health orders.
Pastor Tobias Tissen, who has taken part in several rallies, is currently being sought by the RCMP under an arrest warrant.
The province issued 219 warnings and 131 tickets from May 31 to June 6.
A total of 124 fines of $1,296 were issued to individuals for a total of $160,704.
Five $298 fines were given to people for failure to wear a mask in an indoor public place and one $5,000 ticket was given to a business.
Officials say 116 of the 124 tickets given to individuals were in relation to gatherings in private residences or outdoors.
"Gatherings at private residences continue to be an issue, and will remain a focus of enforcement in the coming weeks to ensure individuals who continue to disregard the public health orders are held to account," they said in their report.
Additional charges are expected as the result of recent large gatherings and rallies.
Manitobans are also being warned that conservation and park patrol officers are enforcing public health and state of emergency orders within provincial parks. Thirty-five people were issued tickets in parks over the past week.
Fines will also increase over time. Unpaid tickets will result in a $100 default conviction penalty on top of the original fine. In those cases the person charged would be prohibited from renewing a driver’s license or vehicle registration until the amount is paid. Unpaid amounts are also sent to a collections agency.
Since enforcement efforts began the province has issued 4,572 warnings and 1,812 tickets resulting in fines totaling over $2.4 million.
Manitobans can report compliance and enforcement issues by visiting www.manitoba.ca/COVID19 or by calling 204-945-3744 or 1-866-626-4862.